CRISPR Explorer
Real reference sequences, guide candidates and live CRISPR research
Education and research planning only, on text. Scores are a simple heuristic, not a validated efficiency or off-target prediction. This page gives no lab procedures and nothing here is for use on a person.
Guide candidates
40
4 genes
CRISPR papers
6
Preprints
5
Trials
0
recruiting, cancer
About the genes
SLC6A4 · solute carrier family 6 member 4 (serotonin transporter) · 17q11.2
This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. It is a target of psychomotor stimulants such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake; results in the literature about any effect on behavior and depression conflict. (NCBI Gene summary, provided by RefSeq, May 2019)
NCBI Gene 6532 · OMIM 182138 · Ensembl · UniProt P31645 · transcript NM_001045.6
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TGCCTTAGCCACCTGAGTAGCTGGGAT✂TACAGGTGTCCACCACTGCCTTAGCCACCTGAGTAGCTGGGAT✂ACAGGTGTCCACCACguide (20) · PAM · ✂ cut, 3 bases before the PAM · deleted
- Length change
- -1 base
- Reading frame (if in a coding region)
- shifted — would scramble the protein downstream
- Guide quality (heuristic)
- GC 50% · score 1.00
Illustration on a 43-base window of the public RefSeq transcript NM_007294.4 (BRCA1). The guide is on the reverse strand, shown 5′→3′. Real repair outcomes are varied and unpredictable; this is not a prediction and not a protocol.
Proof: is this the data the agents logged?
- Digest recomputed here
- f4adb995ebe4c65e7483fa5ff33046ceadaf495246bd26bc3a586e4ccf2b8c63
- Digest the agent logged (chain entry e090f3c42aa7ecd5…)
- f4adb995ebe4c65e7483fa5ff33046ceadaf495246bd26bc3a586e4ccf2b8c63
The agent hashes the guide list when it downloads the sequences and writes that hash into the hash-chained audit trail. This page hashes what is stored and compares. A mismatch means the stored guides changed after they were logged.
Guide candidates
| Gene | Strand · pos | Guide (5′→3′) | PAM | GC | Score | Transcript |
|---|---|---|---|---|---|---|
| BRCA1 | -630 | ACCTGAGTAGCTGGGATTAC | AGG | 50% | 1.00 | NM_007294.4 |
| BRCA1 | -765 | TCCCAAAGTGCTGGGATTAC | AGG | 50% | 1.00 | NM_007294.4 |
| BRCA1 | -1490 | CACACATCTGCCCAATTGCA | TGG | 50% | 1.00 | NM_007294.4 |
| BRCA1 | +1636 | TACATCAGGCCTTCATCCTG | AGG | 50% | 1.00 | NM_007294.4 |
| BRCA1 | -1688 | CCCTGAAGATCTTTCTGTCC | TGG | 50% | 1.00 | NM_007294.4 |
| BRCA1 | -2264 | AGAGGCTGATTCCAGATTCC | AGG | 50% | 1.00 | NM_007294.4 |
| BRCA1 | +600 | GAACTCTGAGGACAAAGCAG | CGG | 50% | 1.00 | NM_007294.4 |
| BRCA1 | +729 | AAATCACCCCTCAAGGAACC | AGG | 50% | 1.00 | NM_007294.4 |
| BRCA1 | +1944 | TGAGGAGGAAGTCTTCTACC | AGG | 50% | 1.00 | NM_007294.4 |
| BRCA1 | +2470 | GGAAGTTAGCACTCTAGGGA | AGG | 50% | 1.00 | NM_007294.4 |
| BRCA2 | -235 | CAGATGGATCACCTGACATC | AGG | 50% | 1.00 | NM_000059.4 |
| BRCA2 | +1797 | TGAAGCCTCTGAAAGTGGAC | TGG | 50% | 1.00 | NM_000059.4 |
| BRCA2 | -1895 | CAAATGGGACTAACAGGTGG | AGG | 50% | 1.00 | NM_000059.4 |
| BRCA2 | -2168 | TTAGTTGGGGTGGACCACTT | GGG | 50% | 1.00 | NM_000059.4 |
| BRCA2 | -3449 | TCAAGAGGTGTACAGGCATC | AGG | 50% | 1.00 | NM_000059.4 |
| BRCA2 | -3967 | TCCAGCCTTTCCATCATTGG | AGG | 50% | 1.00 | NM_000059.4 |
| BRCA2 | +4675 | GAAAGTGTCCCAGTTGGTAC | TGG | 50% | 1.00 | NM_000059.4 |
| BRCA2 | -4741 | AGTTGTGCGAAAGGGTACAC | AGG | 50% | 1.00 | NM_000059.4 |
| BRCA2 | -338 | CCCTGTAATCCCAGCTACTA | GGG | 50% | 1.00 | NM_000059.4 |
| BRCA2 | -4178 | GCTGCTTTCAGAGAGATTCG | AGG | 50% | 1.00 | NM_000059.4 |
| PARP1 | +323 | TTCTCCTGCTTCTGGAAGGT | GGG | 50% | 1.00 | NM_001618.4 |
| PARP1 | +646 | CTGCTTTGTCAAGAACAGGG | AGG | 50% | 1.00 | NM_001618.4 |
| PARP1 | +965 | AACAAGCAGCAAGTGCCTTC | TGG | 50% | 1.00 | NM_001618.4 |
| PARP1 | -1078 | CTGAGACGTATGGCAGTAGT | TGG | 50% | 1.00 | NM_001618.4 |
| PARP1 | +1081 | CGATGCCTATTACTGCACTG | GGG | 50% | 1.00 | NM_001618.4 |
| PARP1 | -1257 | GCTTAAAGGGCTTGTAACGC | TGG | 50% | 1.00 | NM_001618.4 |
| PARP1 | +1510 | CAACATCCGAGTTGTGTCTG | AGG | 50% | 1.00 | NM_001618.4 |
| PARP1 | +472 | TAGCAAGGCAGAGAAGACTC | TGG | 50% | 1.00 | NM_001618.4 |
| PARP1 | +785 | AGAAAAGGCGATGAGGTGGA | TGG | 50% | 1.00 | NM_001618.4 |
| PARP1 | +1390 | GGCCATGATTGAGAAACTCG | GGG | 50% | 1.00 | NM_001618.4 |
| SLC6A4 | +443 | TACTCAGCAGTTCCAAGTCC | TGG | 50% | 1.00 | NM_001045.6 |
| SLC6A4 | +928 | CTGCACCAATTACTTCTCCG | AGG | 50% | 1.00 | NM_001045.6 |
| SLC6A4 | -1063 | TCCCAAGTAGCTGGGATTAC | AGG | 50% | 1.00 | NM_001045.6 |
| SLC6A4 | +1246 | ACCCAATTGGCAGAAACTCC | TGG | 50% | 1.00 | NM_001045.6 |
| SLC6A4 | +1409 | TGCATGACGAGCTTCGTTTC | GGG | 50% | 1.00 | NM_001045.6 |
| SLC6A4 | -1523 | GAGTTTCGCTCTTGTCATCC | AGG | 50% | 1.00 | NM_001045.6 |
| SLC6A4 | +1709 | GTGGTCATCACCTGCTTCTT | TGG | 50% | 1.00 | NM_001045.6 |
| SLC6A4 | +335 | CAGCTATCAGCGTGTGAAGA | TGG | 50% | 1.00 | NM_001045.6 |
| SLC6A4 | +519 | CTGAGCTTCATCAAGGGGAA | CGG | 50% | 1.00 | NM_001045.6 |
| SLC6A4 | +829 | CTACAACACCATCATGGCCT | GGG | 50% | 1.00 | NM_001045.6 |